Immuno-Based Molecular Scaffolding of Glucose Dehydrogenase and Ferrocene Mediator on fd Viral Particles Yields Enhanced Bioelectrocatalysis ACS Paragon Plus Environment

Kristian Torbensen (1) , Anisha Patel (1) , Agnès Anne (1) , Christophe Demaille (1) , Laure Bataille (2) , Thierry Michon (2) , Eric Grelet (3)

1 LEM (UMR_7591) - Laboratoire d'Electrochimie Moléculaire
2 BFP - Biologie du fruit et pathologie
3 CRPP - Centre de Recherche Paul Pascal

A virus-based nanostructuring strategy is proposed for improving the catalytic performance of integrated redox enzyme electrodes. Random arrays of adsorbed filamentous fd bacteriophage particles, used as scaffolds, are assembled onto gold electrode surfaces. The viral particles are endowed with functionally coupled enzymatic and redox properties, by the sequential immunological assembly of quinoprotein glucose dehydrogenase conjugated antibodies and ferrocene PEGylated antibodies on their protein shell. The resulting virus-scaffolded enzyme/redox mediator integrated system displays a large enhancement in the catalytic current generated per enzyme molecule (i.e., in enzymatic turnover) as compared with nonscaffolded integrated glucose oxidizing enzyme electrodes. The mechanism underlying the observed scaffolding-induced catalytic enhancement is deciphered. Confinement of the mediator on the viral scaffold enables fast electron transport rate and shifts the enzyme behavior into its most effective cooperative kinetic mode.

Mots clés: bioelectrocatalytic viral particles; enzyme; bioelectrocatalysis; pyrroloquinoline quinone-dependent glucose dehydrogenase; nanocarriers; bioscaffolding

Domaines: Chimie; Chimie analytique; Catalyse; Chimie théorique et/ou physique

Kristian Torbensen, Anisha Patel, Agnès Anne, Christophe Demaille, Laure Bataille, et al.. Immuno-Based Molecular Scaffolding of Glucose Dehydrogenase and Ferrocene Mediator on fd Viral Particles Yields Enhanced Bioelectrocatalysis ACS Paragon Plus Environment. ACS Catalysis, 2019, 9 (6), pp.5783-5796. ⟨10.1021/acscatal.9b01263⟩. ⟨hal-02186135⟩