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Exploring the role of NCCR variation on JC polyomavirus expression from dual reporter minicircles

Abstract : JC virus (JCV), a ubiquitous human polyomavirus, can cause fatal progressive multifocal leukoencephalopathy (PML) in immune compromised patients. The viral genome is composed of two conserved coding regions separated by a highly variable non-coding control region (NCCR). We analyzed the NCCR sequence from 10 PML JCV strains and found new mutations. Remarkably, the NCCR f section was mutated in most cases. We therefore explored the importance of this section in JCV expression in renal (HEK293H) and glioblastoma (U-87MG) cell lines, by adapting the emerging technology of DNA minicircles. Using bidirectional fluorescent reporters, we revealed that impaired NCCR-driven late expression in glioblastoma cells was restored by a short deletion overlapping e and f sections. This study evidenced a relevant link between JCV NCCR polymorphism and cell-type dependent expression. The use of DNA minicircles opens new insights for monitoring the impact of NCCR variation.
Keywords : MICROBIO ACTINO
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https://hal.archives-ouvertes.fr/hal-02180801
Contributor : Marie-Claude Serre <>
Submitted on : Thursday, July 11, 2019 - 4:05:51 PM
Last modification on : Thursday, July 8, 2021 - 3:51:56 AM

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Anne-Sophie l'Honneur, Hervé Leh, Fanny Laurent-Tchenio, Uriel Hazan, Flore Rozenberg, et al.. Exploring the role of NCCR variation on JC polyomavirus expression from dual reporter minicircles. PLoS ONE, Public Library of Science, 2018, 13 (6), pp.e0199171. ⟨10.1371/journal.pone.0199171⟩. ⟨hal-02180801⟩

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