Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs

Graciela M. Dias (1, 2) , Adeline Bidault (1) , Patrick Le Chevalier (3) , Gwenaëlle Choquet (1) , Clio Der Sarkissian (4) , Ludovic Orlando (4, 5) , Claudine Médigue (6) , Valerie Barbe (7) , Sophie Mangenot (7) , Cristiane C. Thompson (2) , Fabiano L. Thompson (2) , Annick Jacq (8, 9, 10) , Vianney Pichereau (1) , Christine Paillard (1)

1 LEMAR - Laboratoire des Sciences de l'Environnement Marin (LEMAR)
2 UFRRJ - Universidade Federal Rural do Rio de Janeiro
3 LBCM - Laboratoire de Biotechnologie et Chimie Marines
4 Section for GeoGenetics
5 AMIS - Anthropologie Moléculaire et Imagerie de Synthèse
6 LABGeM - Analyse Bio-Informatique pour la Génomique et le Métabolisme
7 GENOSCOPE - Genoscope - Centre national de séquençage [Evry]
8 I2BC - Institut de Biologie Intégrative de la Cellule
9 SRRB - Signalisation et Réseaux de Régulations Bactériens
10 DBG - Département Biologie des Génomes

The Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, , which is also pathogenic to fish. Here we report the first assembly of the complete genome of CECT4600, together with the genome sequences of 16 additional strains isolated across a broad host and geographic range. Our extensive genome dataset allowed us to describe the pathogen pan- and core genomes and to identify putative virulence factors. The core genome consists of 3,352 genes, including multiple potential virulence factors represented by haemolysins, transcriptional regulators, Type I restriction modification system, GGDEF domain proteins, several conjugative plasmids, and a Type IV secretion system. Future research on the coevolutionary arms race between virulence factors and host resistance mechanisms will improve our understanding of how pathogenicity develops in this emerging pathogen.

Mots clés: ACL; Venerupis philippinarum; Vibrio tapetis; core genome; comparative genomics; pangenome; pathogenicity; T4SS

Domaines: Sciences du Vivant [q-bio]; Biodiversité et Ecologie

Graciela M. Dias, Adeline Bidault, Patrick Le Chevalier, Gwenaëlle Choquet, Clio Der Sarkissian, et al.. Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs. Frontiers in Microbiology, 2018, 9, pp.227. ⟨10.3389/fmicb.2018.00227⟩. ⟨hal-02177600⟩