Adaptive optics light-sheet microscopy based on direct wavefront sensing without any guide star

Antoine Hubert (1) , Fabrice Harms (2) , Remy Juvénal (3) , Pauline Treimany (1) , Xavier Levecq (3) , Vincent Loriette (4) , Georges Farkouh (5) , François Rouyer (5) , Alexandra Fragola (1)

1 LPEM - Laboratoire de Physique et d'Etude des Matériaux (UMR 8213)
2 IL - Institut Langevin - Ondes et Images (UMR7587)
3 Imagine Optic
4 LP2N - Laboratoire Photonique, Numérique et Nanosciences
5 NeuroPSI - Institut des Neurosciences Paris-Saclay

We propose an Adaptive Optics Light-Sheet Fluorescence Microscope (AO-LSFM) for closed-loop aberrations correction at the emission path, providing intrinsic instrumental simplicity and high accuracy when compared to previously reported schemes. The approach is based on direct wavefront sensing i.e. not on time-consuming iterative algorithms, and does not require the use of any guide star thus reducing instrumental complexity and/or sample preparation constraints. The design is based on a modified Shack-Hartmann wavefront sensor providing compatibility with extended sources such as images from optical sectioning microscopes. We report an AO-LSFM setup based on such sensor including characterization of the sensor performance, and demonstrate for the first time significant contrast improvement on neuronal structures of the ex-vivo adult drosophila brain in depth.

Domaines: Optique [physics.optics]

Antoine Hubert, Fabrice Harms, Remy Juvénal, Pauline Treimany, Xavier Levecq, et al.. Adaptive optics light-sheet microscopy based on direct wavefront sensing without any guide star. Optics Letters, 2019, 44 (10), pp.2514. ⟨10.1364/OL.44.002514⟩. ⟨hal-02163939⟩