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A novel plasmid‐transcribed regulatory sRNA, QfsR, controls chromosomal polycistronic gene expression in Agrobacterium fabrum

Abstract : Plasmids are mobile DNAs that can adjust host cell functions for their own amplification and dissemination. We identified Quorum sensing flagella small RNA regulator (QfsR), a small RNA, transcribed from the virulence tumour-inducing (Ti) plasmid in the phytopathogen Agrobacterium fabrum. QfsR is widely conserved throughout RepABC plasmids carried by Rhizobiaceae. Target prediction, expression analysis and site-direct mutagenesis experiments showed that QfsR directly pairs within polycistronic mRNAs transcribed from chromosome (genes involved in flagella apparatus) and Ti plasmid (genes involved in conjugative transfer). QfsR leads to a coordinated expression of whole polycistronic mRNA molecules. Whereas a lack of QfsR represses motility, its overproduction increases the quorum sensing signal accumulation and the Ti plasmid conjugative transfer. Based on these observations, we propose QfsR as a hub connecting regulatory networks of motility and plasmid conjugative transfer. To our knowledge, QfsR is the first example of a plasmid-encoded sRNA that controls chromosomal polycistronic gene expression.
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https://hal.archives-ouvertes.fr/hal-02161890
Contributor : Agnès Rodrigue <>
Submitted on : Friday, June 21, 2019 - 11:18:29 AM
Last modification on : Friday, December 11, 2020 - 12:34:03 PM

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Benjamin Diel, Magali Dequivre, Florence Wisniewski-Dyé, Ludovic Vial, Florence Hommais. A novel plasmid‐transcribed regulatory sRNA, QfsR, controls chromosomal polycistronic gene expression in Agrobacterium fabrum. Environmental Microbiology, Wiley-Blackwell, 2019, 21 (8), pp.3063-3075. ⟨10.1111/1462-2920.14704⟩. ⟨hal-02161890⟩

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