Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Virology Année : 1989

Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo.

J Bol
  • Fonction : Auteur
A Astier-Manifacier
  • Fonction : Auteur
A. L. Haenni
  • Fonction : Auteur
Gargouri R.
  • Fonction : Auteur

Résumé

Turnip yellow mosaic virus (TYMV) possesses a monopartite single-stranded (+) sense RNA genome in which the coat protein (cp) gene is 3' proximal and is expressed in vivo via a subgenomic RNA. Evidence is presented here that this subgenomic RNA is synthesized in vivo by internal initiation of replication on (-) RNA strands of genomic length. The double-stranded RNAs (dsRNAs) from TYMV-infected plants have been isolated, purified, and characterized. Under native conditions, no dsRNAs (replicative intermediates and/or replicative forms) of subgenomic length corresponding to subgenomic cp RNA can be detected by ethidium bromide staining of RNA-sizing gels or by Northern blot hybridization using RNA probes. The presence of nascent subgenomic cp (+) RNA strands on the dsRNA of genomic length has been demonstrated using two different approaches: (1) Northern blot hybridization using (-) RNA probes under denaturing conditions and (2) characterization of the 5' ends of nascent (+) RNA strands upon labeling by vaccinia virus nucleoside-2'-methyltransferase.
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Dates et versions

hal-02160173 , version 1 (19-06-2019)

Identifiants

  • HAL Id : hal-02160173 , version 1
  • PUBMED : 2763458

Citer

Rajiv L Joshi, J Bol, A Astier-Manifacier, A. L. Haenni, Gargouri R.. Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo.. Virology, 1989, 171 (2), pp.386-93. ⟨hal-02160173⟩
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