Distinct Distribution of Ectopically Expressed Histone Variants H2A.Bbd and MacroH2A in Open and Closed Chromatin Domains

Elena Ioudinkova (1, 2) , Ana Barat (2, 3) , Andrey A. Pichugin (2) , Elena Markova (1, 2) , Ilya Sklyar (1, 2) , Iryna Pirozhkova (2) , Chloé Robin (2) , Marc Lipinski (2) , Vasily Ogryzko (2) , Yegor Vassetzky (2) , Sergey Razin (1)

1 Institute of Gene Biology, Russian Academy of Sciences
2 UMR8126 - Signalisation, noyaux et innovations en cancérologie
3 School of Computing [Dublin]

It becomes increasingly evident that nuclesomes are far from being identical to each other. This nucleosome diversity is due partially to the existence of histone variants encoded by separate genes. Among the known histone variants the less characterized are H2A.Bbd and different forms of macroH2A. This is especially true in the case of H2A.Bbd as there are still no commercially available antibodies specific to H2A.Bbd that can be used for chromatin immunoprecipitation (ChIP).

Domaines: Sciences du Vivant [q-bio]

Elena Ioudinkova, Ana Barat, Andrey A. Pichugin, Elena Markova, Ilya Sklyar, et al.. Distinct Distribution of Ectopically Expressed Histone Variants H2A.Bbd and MacroH2A in Open and Closed Chromatin Domains. PLoS ONE, 2012, 7 (10), pp.e47157. ⟨10.1371/journal.pone.0047157⟩. ⟨hal-02159968⟩