Skip to Main content Skip to Navigation
Journal articles

Phenotyping Thermal Responses of Yeasts and Yeast-like Microorganisms at the Individual and Population Levels: Proof-of-Concept, Development and Application of an Experimental Framework to a Plant Pathogen

Abstract : Deciphering the responses of microbial populations to spatiotemporal changes in their thermal environment is instrumental in improving our understanding of their eco-evolutionary dynamics. Recent studies have shown that current phenotyping protocols do not adequately address all dimensions of phenotype expression. Therefore, these methods can give biased assessments of sensitivity to temperature, leading to misunderstandings concerning the ecological processes underlying thermal plasticity. We describe here a new robust and versatile experimental framework for the accurate investigation of thermal performance and phenotypic diversity in yeasts and yeast-like microorganisms, at the individual and population levels. In addition to proof-of-concept, the application of this framework to the fungal wheat pathogen Zymoseptoria tritici resulted in detailed characterisations for this yeast-like microorganism of (i) the patterns of temperature-dependent changes in performance for four fitness traits; (ii) the consistency in thermal sensitivity rankings of strains between in planta and in vitro growth assessments; (iii) significant interindividual variation in thermal responses, with four principal thermotypes detected in a sample of 66 strains; and (iv) the ecological consequences of this diversity for population-level processes through pairwise competition experiments highlighting temperature-dependent outcomes. These findings extend our knowledge and ability to quantify and categorise the phenotypic heterogeneity of thermal responses. As such, they lay the foundations for further studies elucidating local adaptation patterns and the effects of temperature variations on eco-evolutionary and epidemiological processes.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-02159596
Contributor : Archive Ouverte Prodinra <>
Submitted on : Wednesday, August 12, 2020 - 2:36:57 PM
Last modification on : Tuesday, June 15, 2021 - 2:57:23 PM
Long-term archiving on: : Monday, November 30, 2020 - 6:48:58 PM

File

2019 - Boixel-post-print-Micro...
Files produced by the author(s)

Identifiers

Citation

Anne-Lise Boixel, Ghislain Delestre, Jean Legeay, Michaël Chelle, Frederic Suffert. Phenotyping Thermal Responses of Yeasts and Yeast-like Microorganisms at the Individual and Population Levels: Proof-of-Concept, Development and Application of an Experimental Framework to a Plant Pathogen. Microbial Ecology, Springer Verlag, 2019, 78 (1), pp.42-56. ⟨10.1007/s00248-018-1253-6⟩. ⟨hal-02159596⟩

Share

Metrics

Record views

187

Files downloads

126