The Effect of Viral Regulatory Protein Expression on Gene Delivery by Human Immunodeficiency Virus Type 1 Vectors Produced in Stable Packaging Cell Lines

Narasimhachar Srinivasakumar (1) , Nathalie Chazal (2) , C Helga-Maria (1) , Susan Prasad (1) , Marie-Louise Hammarskjöld (1) , David Rekosh (1)

1 University of Virginia
2 IRIM - Institut de Recherche en Infectiologie de Montpellier

We describe the generation of stable human immunodeficiency virus type 1 (HIV-1)-packaging lines that constitutively express high levels of HIV-1 structural proteins in either a Rev-dependent or a Rev-independent fashion. These cell lines were used to assess gene transfer by using an HIV-1 vector expressing the hygromycin B resistance gene and to study the effects of Rev, Tat, and Nef on the vector titer. The Rev-independent cell lines were created by using gag-pol and env expression vectors that contain the Mason-Pfizer monkey virus (MPMV) constitutive transport element (CTE). Vector titers approaching 10 4 CFU/ml were routinely obtained with these cell lines, as well as with the Rev-dependent cell lines, with HeLa-CD4 cells as targets. The presence of Nef and Tat in the producer cell each increased the vector titer 5-to 10-fold. Rev, on the other hand, was absolutely essential for gene transfer, unless the MPMV CTE was present in the vector. In that case, by using the Rev-independent cell lines for packaging, Rev could be completely eliminated from the system without a reduction in vector titer.

Domaines: Virologie

Narasimhachar Srinivasakumar, Nathalie Chazal, C Helga-Maria, Susan Prasad, Marie-Louise Hammarskjöld, et al.. The Effect of Viral Regulatory Protein Expression on Gene Delivery by Human Immunodeficiency Virus Type 1 Vectors Produced in Stable Packaging Cell Lines. Journal of Virology, 1997, 71 (8), pp.5841-5848. ⟨hal-02147239⟩