Epitopes of the 44-68 human parathyroid hormone fragments: the importance of specific hydrophilic peptide sequences.

A. Delmas (1) , G Milhaud (1) , D Raulais (1) , P Rivaille (1)

1 CHU Saint-Antoine [AP-HP]

Several pentapeptides included in the 44-68 sequence of human parathyroid hormone (hPTH) were synthesized simultaneously on benzhydrylamine and m-nitrobenzhydrylamine resins. The first polymer gave the free peptide and the second the peptidyl-resin complex. An ELISA test carried out with each peptidyl-resin complex showed that all the anti-44-68 hPTH antibodies raised in different animal species are directed against the same hPTH pentapeptidic sequence. This sequence is very hydrophilic and is specific to the hormone. This study demonstrates the importance of specific peptide chains in an epitope.

Domaines: Sciences du Vivant [q-bio]

A. Delmas, G Milhaud, D Raulais, P Rivaille. Epitopes of the 44-68 human parathyroid hormone fragments: the importance of specific hydrophilic peptide sequences.. Molecular Immunology, 1985, 22 (6), pp.675-9. ⟨10.1016/0161-5890(85)90097-5⟩. ⟨hal-02146974⟩