Synthesis and fluorescence properties of Oregon Green 514 labeled peptides.

C Delmotte (1) , A. Delmas (1)

1 CBM - Centre de biophysique moléculaire

Oregon Green 514, with high photostability and a pKa of 4.8, is suitable for imaging applications. Labeling of peptide-bound resin with the carboxylic acid form was optimized. Peptide conjugation resulted in altered fluorescence properties of the dye including a quenching of the intensity compared with that in aqueous buffers with a slight red-shift of the emission maximum.

Domaines: Sciences du Vivant [q-bio]

C Delmotte, A. Delmas. Synthesis and fluorescence properties of Oregon Green 514 labeled peptides.. Bioorganic and Medicinal Chemistry Letters, 1999, 9 (20), pp.2989-94. ⟨hal-02146730⟩