Deciphering the reading of the genetic code by near-cognate tRNA - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2018

Deciphering the reading of the genetic code by near-cognate tRNA

Résumé

Some codons of the genetic code can be read not only by cognate, but also by near-cognate tRNAs. This flexibility is thought to be conferred mainly by a mismatch between the third base of the codon and the first of the anticodon (the so-called "wobble" position). However, this simplistic explanation underestimates the importance of nucleotide modifications in the decoding process. Using a system in which only near-cognate tRNAs can decode a specific codon, we investigated the role of six modifications of the anticodon, or adjacent nucleotides, of the tRNAs specific for Tyr, Gln, Lys, Trp, Cys, and Arg in Saccharomyces cerevisiae. Modifications almost systematically rendered these tRNAs able to act as near-cognate tRNAs at stop codons, even though they involve noncanonical base pairs, without markedly affecting their ability to decode cognate or near-cognate sense codons. These findings reveal an important effect of modifications to tRNA decoding with implications for understanding the flexibility of the genetic code. translation fidelity | stop codon readthrough | tRNA modification | genetic code | near-cognate tRNA
Fichier principal
Vignette du fichier
1715578115.full.pdf (917.33 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02145351 , version 1 (04-06-2019)

Identifiants

Citer

Sandra Blanchet, David Cornu, Isabelle Hatin, Henri Grosjean, Pierre Bertin, et al.. Deciphering the reading of the genetic code by near-cognate tRNA. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115 (12), pp.3018-3023. ⟨10.1073/pnas.1715578115⟩. ⟨hal-02145351⟩
56 Consultations
225 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More