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Journal articles
An histidine covalent receptor and butenolide complex mediates strigolactone perception
Alexandre de Saint Germain
1
Guillaume Clavé
2
Marie-Ange Badet-Denisot
3
Jean-Paul Pillot
4
David Cornu
5
Jean-Pierre Le Caër
3
Marco Burger
6
Frank Pelissier
3
Pascal Retailleau
3
Colin Turnbull
7
Sandrine Bonhomme
8
Joanne Chory
9
Catherine Rameau
8
François-Didier Boyer
3
Alexandre A. de Saint Germain 1
Author
Guillaume Clavé 2
Author IdHAL : guillaume-clave ORCID : https://orcid.org/0000-0001-6401-0935
Marie-Ange Badet-Denisot 3
Author
Jean-Paul Pillot 4
Author
Pascal ́ Retailleau 3
Author
Colin Turnbull 7
Author
Sandrine Bonhomme 8
Author
Joanne Chory 9
Author
Catherine Rameau 8
Author
François-Didier Boyer 3
Author
1
IJPB - Institut Jean-Pierre Bourgin (INRA Centre de Versailles-Grignon, Route de St-Cyr (RD10), 78026 Versailles Cedex - France)
- INRA - Institut National de la Recherche Agronomique : UMR1318 (France)
- AgroParisTech (16 rue Claude Bernard F-75231 Paris Cedex 05 - France)
2
ICSN - Institut de Chimie des Substances Naturelles (CNRS, Bâtiment 27, Avenue de la Terrasse, 91198, Gif-sur-Yvette cedex - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- CNRS - Centre National de la Recherche Scientifique : UPR 2301 (France)
3
ICSN - Institut de Chimie des Substances Naturelles (Avenue de la terrasse 91198 Gif sur yvette cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UPR2301 (France)
4
ISM - Institut des Sciences Moléculaires (Bâtiment A 12 351 cours de la Libération 33405 TALENCE CEDEX - France)
- Université Montesquieu - Bordeaux 4 (Avenue Léon Duguit - 33608 Pessac cedex - France)
- Université Sciences et Technologies - Bordeaux 1 (351 cours de la Libération - 33405 Talence cedex - France)
- École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB) (France)
- CNRS - Centre National de la Recherche Scientifique : UMR5255 (France)
5
SICaPS - SICaPS (I2BC, Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex
- France)
- PF I2BC - Département Plateforme (I2BC, Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/I2BC (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 9198 (France)
- I2BC - Institut de Biologie Intégrative de la Cellule (Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex - France)
6
The Salk Institute for Biological Studies (10010 N Torrey Pines Rd, La Jolla, CA 92037, États-Unis - United States)
7
Division of cell and molecular biology (London - United Kingdom)
- Imperial College London (South Kensington Campus, London SW7 2AZ - United Kingdom)
8
UR254 - Unité de Recherche en Génétique et Amélioration des Plantes (Route de Saint-Cyr 78026 VERSAILLES Cedex - France)
- INRA - Institut National de la Recherche Agronomique : UR254 (France)
9
Plant Biology Laboratory and Howard Hughes Medical Institute (10010 North Torrey Pines Road, La Jolla, CA 92037, USA - United States)
- The Salk Institute for Biological Studies (10010 N Torrey Pines Rd, La Jolla, CA 92037, États-Unis - United States)
Abstract : Strigolactone plant hormones control plant architecture and are key players in both symbiotic and parasitic interactions. They contain an ABC tricyclic lactone connected to a butenolide group, the D ring. The DWARF14 (D14) strigolactone receptor belongs to the superfamily of α/β-hydrolases, and is known to hydrolyze the bond between the ABC lactone and the D ring. Here we characterized the binding and catalytic functions of RAMOSUS3 (RMS3), the pea (Pisum sativum) ortholog of rice (Oryza sativa) D14 strigolactone receptor. Using new profluorescent probes with strigolactone-like bioactivity, we found that RMS3 acts as a single-turnover enzyme that explains its apparent low enzymatic rate. We demonstrated the formation of a covalent RMS3-D-ring complex, essential for bioactivity, in which the D ring was attached to histidine 247 of the catalytic triad. These results reveal an undescribed mechanism of plant hormone reception in which the receptor performs an irreversible enzymatic reaction to generate its own ligand.
Document type :
Journal articles
Complete list of metadatas
https://hal.sorbonne-universite.fr/hal-02144648
Contributor : Marie-Ange Badet-Denisot <>
Submitted on : Friday, May 31, 2019 - 10:46:13 AM
Last modification on : Friday, November 6, 2020 - 12:32:02 PM
Contributor : Marie-Ange Badet-Denisot <>
Submitted on : Friday, May 31, 2019 - 10:46:13 AM
Last modification on : Friday, November 6, 2020 - 12:32:02 PM
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Identifiers
- HAL Id : hal-02144648, version 1
- DOI : 10.1038/nchembio.2147
- PRODINRA : 379092
- PUBMED : 27479744
- WOS : 000384248600009
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Citation
Alexandre de Saint Germain, Guillaume Clavé, Marie-Ange Badet-Denisot, Jean-Paul Pillot, David Cornu, et al.. An histidine covalent receptor and butenolide complex mediates strigolactone perception. Nature Chemical Biology, Nature Publishing Group, 2016, 12 (10), pp.787-794. ⟨10.1038/nchembio.2147⟩. ⟨hal-02144648⟩
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