A new method for the determination of Leuconostoc mesenteroides cell number

Hélène Lange (1) , Florence Plihon (1) , Patricia Taillandier (1) , Jean-Pierre Riba (1)

1 LGC - Laboratoire de Génie Chimique

Dilution needed for plate counts (CFU/ml) is known to disrupt streptococci chains, and therefore the validity of this method is controversial. A novel apparatus was designed to disrupt Leuconostoc mesenteroides chains in pairs, by forcing them to pass through a narrow capillary tube. When Leuconostoc mesenteroides occurs in pairs it appears that the real cell concentration can be simply deduced by doubling the CFU/ml.

Mots clés: Leuconostoc mesenteroides; Chain disruption; Plate count; Cell concentration

Domaines: Génie chimique; Génie des procédés

Hélène Lange, Florence Plihon, Patricia Taillandier, Jean-Pierre Riba. A new method for the determination of Leuconostoc mesenteroides cell number. Journal of Microbiological Methods, 1995, 24 (1), pp.93-97. ⟨10.1016/0167-7012(95)00058-5⟩. ⟨hal-02143235⟩