Characterization of a continuous smooth muscle cell line derived from rabbit aorta.

Abstract : A spontaneously arising continuous cell line (Rb-1) derived from collagenase-elastase digested rabbit aorta has been propagated in vitro for over 100 passages. During this period, the Rb-1 cells remained spindle-shaped and formed regularly oriented parallel bundles. After Passage 50, Rb-1 cells were found to be serum-independent in their growth and reached higher saturation density than rabbit aorta smooth muscle cells. Alpha-actin and desmin filaments were detected by immunostaining in Rb-1 cells and early passage of rabbit aorta smooth muscle cells. The proportion of alpha-actin transcripts in Rb-1 cells was lower than that of transcripts for beta- and gamma-actins. The modal chromosome number was maintained at 44 between Passages 11 and 60, and two marker chromosomes were constantly present. Infection of Rb-1 cells with two strains of herpes simplex virus type 1 resulted in high titers of virus, whereas a herpes simplex virus type 2 temperature-sensitive mutant replicated only at the permissive temperature. The Rb-1 cell line could be used for the study of vascular smooth muscle cell proliferation and their interaction with viruses.
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M Nachtigal, M Nagpal, P Greenspan, A Nachtigal, Alain Legrand. Characterization of a continuous smooth muscle cell line derived from rabbit aorta.. In vitro cellular & developmental biology : journal of the Tissue Culture Association, 1989, 25 (10), pp.892-8. ⟨10.1007/BF02624001⟩. ⟨hal-02138476⟩



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