Purification, Cloning, Characterization and Essential Amino Acid Residues Analysis of a New i-Carrageenase from Cellulophaga sp. QY3

Su Ma (1) , Gaofei Duan (1) , Wengang Chai (1) , Cunliang Geng (2) , Yulong Tan (1) , Lushan Wang (2) , Frédéric Le Sourd (3) , Gurvan Michel (3) , Wengong Yu (1) , Feng Han (1)

1 OUC - Ocean University of China
2 Shandong University
3 LBI2M - Laboratoire de Biologie Intégrative des Modèles Marins

i-Carrageenases belong to family 82 of glycoside hydrolases that degrade sulfated galactans in the red algae known as i-carrageenans. The catalytic mechanism and some substrate-binding residues of family GH82 have been studied but the substrate recognition and binding mechanism of this family have not been fully elucidated. We report here the purification, cloning and characterization of a new i-carrageenase CgiA_Ce from the marine bacterium Cellulophaga sp. QY3. CgiA_Ce was the most thermostable carrageenase described so far. It was most active at 50uC and pH 7.0 and retained more than 70% of the original activity after incubation at 50uC for 1 h at pH 7.0 or at pH 5.0-10.6 for 24 h. CgiA_Ce was an endo-type i-carrageenase; it cleaved i-carrageenan yielding neo-i-carrabiose and neo-i-carratetraose as the main end products, and neo-i-carrahexaose was the minimum substrate. Sequence analysis and structure modeling showed that CgiA_Ce is indeed a new member of family GH82. Moreover, sequence analysis of i-carrageenases revealed that the amino acid residues at subsites 21 and +1 were more conserved than those at other subsites. Site-directed mutagenesis followed by kinetic analysis identified three strictly conserved residues at subsites 21 and +1 of i-carrageenases, G228, Y229 and R254 in CgiA_Ce, which played important roles for substrate binding. Furthermore, our results suggested that Y229 and R254 in CgiA_Ce interacted specifically with the sulfate groups of the sugar moieties located at subsites 21 and +1, shedding light on the mechanism of i-carrageenan recognition in the family GH82.

Domaines: Biochimie [q-bio.BM]

Su Ma, Gaofei Duan, Wengang Chai, Cunliang Geng, Yulong Tan, et al.. Purification, Cloning, Characterization and Essential Amino Acid Residues Analysis of a New i-Carrageenase from Cellulophaga sp. QY3. PLoS ONE, 2013, 8 (5), pp.e64666. ⟨10.1371/journal.pone.0064666⟩. ⟨hal-02138205⟩