Glycofection: The Ubiquitous Roles of Sugar Bound on Glycoplexes

Abstract : Glycofection (transfection by using sugar-substituted polylysine) was assessed in order to provide an alternative to viral vectors for the transfer of genes into vascular smooth muscle cells. A rabbit vascular smooth muscle cell line (Rb-1 cells) was selectively transfected by using glycoplexes (glycosylated polylysine/pSV2LUC complexes) in the presence of 10 mu M of the fusogenic peptide GALA. A sugar-specific transfection was obtained when the glycofection was conducted for 1 h with glycoplexes containing either alpha Gal, alpha -Glc, alpha -GalNAc, beta -GlcNAc, or beta -GalNAc residues. The gene expression was high after transfection, with glycoplexes bearing alpha Gal, alpha -GalNAc, or beta -GalNAc residues that were weakly internalized, and low with glycoplexes carrying Lact or Rha residues that were well taken up by cells. These results suggest that 1) glycofection can be a good approach for a selective transfer of genes intovascular smooth muscle cells, 2) an efficient uptake of the glycoplexes is not the unique limiting step for an efficient transfection, and 3) the sugar-dependent trafficking of the glycoplexes inside the cells may account for the transfection efficiency.
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Valerie Boutin, Alain Legrand, Roger Mayer, Maurice Nachtigal, Michel Monsigny, et al.. Glycofection: The Ubiquitous Roles of Sugar Bound on Glycoplexes. Drug Delivery, Taylor & Francis, 2008, 6 (1), pp.45-50. ⟨10.1080/107175499267156⟩. ⟨hal-02138035⟩



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