Marek’s disease virus (MDV) homologues of herpes simplex virus type 1 UL49 (VP22) and UL48 (VP16) genes: high-level expression and characterization of MDV-1 VP22 and VP16

Abstract : Genes UL49 and UL48 of Marek's disease virus 1 (MDV-1) strain RB1B, encoding the respective homologues of herpes simplex virus type 1 (HSV-1) genes VP22 and VP16, were cloned into a baculovirus vector. Seven anti-VP22 MAbs and one anti-VP16 MAb were generated and used to identify the tegument proteins in cells infected lytically with MDV-1. The two genes are known to be transcribed as a single bicistronic transcript, and the detection of only one of the two proteins (VP22) in MSB-1 lymphoma and in chicken embryo skin cells infected with MDV-1 prompted the study of the transcription/translation of the UL49-48 sequence in an in vivo and in vitro expression system. VP16 was expressed in vitro at detectable levels, whereas it could only be detected at a lower level in a more controlled environment. It was demonstrated that VP22 is phosphorylated in insect cells and possesses the remarkable property of being imported into all cells in a monolayer. VP22 localized rapidly and efficiently to nuclei, like its HSV-1 counterpart. The DNA-binding property of VP22 is also reported and a part of the region responsible for this activity was identified between aa 16 and 37 in the N-terminal region of the protein.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-02135997
Contributor : Laëtitia Legoupil <>
Submitted on : Tuesday, May 21, 2019 - 4:46:23 PM
Last modification on : Friday, May 24, 2019 - 11:36:07 AM

Links full text

Identifiers

Collections

Citation

Fabien Dorange, Slimane El Mehdaoui, Chantal Pichon, Pierre Coursaget, Jean-François Vautherot. Marek’s disease virus (MDV) homologues of herpes simplex virus type 1 UL49 (VP22) and UL48 (VP16) genes: high-level expression and characterization of MDV-1 VP22 and VP16. Journal of General Virology, Microbiology Society, 2000, 81 (9), pp.2219-2230. ⟨10.1099/0022-1317-81-9-2219⟩. ⟨hal-02135997⟩

Share

Metrics

Record views

10