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Improvement of a fed-batch process for high level xylanase production by a Bacillus strain

Abstract : In this paper, the improvement of a fed-batch fermentation from the point of view of an industrial xylanase production process is described. The Bacillus strain chosen for this study is able to produce high quantities of a xylanase that is suitable to be used as bleach boost agent in chlorine-free bleaching sequences of paper pulp. It was found that xylo-oligosaccharides hydrolysis products from xylan by xylanase action were indispensable for induction of the enzyme synthesis, but that their presence in quantities of only 0.1g dm^-3 xylose equivalents led to catabolite repression. A substrate-limited fed-batch process, that is the most adapted, was furthermore improved with regard to nutrient requirement of the microorganism, especially the nitrogen source. A process with constant supply of a culture medium containing xylan, peptone and mineral nitrogen was able to produce 20240nkatcm^-3 with a productivity of 910nkatcm^-3 h^-1, which places the process among the best ever reported.
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Gerhard Schneider, Pierre Strehaiano, Patricia Taillandier. Improvement of a fed-batch process for high level xylanase production by a Bacillus strain. Journal of Chemical Technology and Biotechnology, Wiley, 2001, 76 (5), pp.456-460. ⟨10.1002/jctb.415⟩. ⟨hal-02135100⟩



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