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Synthesis of nc-UO 2 by controlled precipitation in aqueous phase

Abstract : A new strain of actinobacteria, designated ACD1, was isolated from a Saharan soil sample in the Hoggar region (Algeria). Morphological study led to this strain being classified as a member of the Actinomadura genus. Phylogenetic analysis based on the 16S rRNA gene showed that the strain is closely related to Actinomadura sediminis DSM 45500T (98.5% sequence similarity). Furthermore, strain ACD1 presented a strong activity against mycotoxigenic and phytopathogenic fungi, including Aspergillus and Fusarium strains, and other pathogenic microorganisms. The kinetics of antimicrobial activity were investigated on ISP-2, Bennett and TSB media. Four solvents (n-hexane, dichloromethane, ethyl acetate and n-butanol) were used for the extraction of the produced antibiotic. The highest antimicrobial activity was obtained using the butanolic extract from the ISP-2 medium after seven days of fermentation culture. The active antibiotic was purified by reverse-phase HPLC using a C18 column. The UV-visible and mass spectra were determined. The minimum inhibitory concentrations (MIC) of this antibiotic were determined against pathogenic microorganisms.
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Submitted on : Monday, May 20, 2019 - 5:38:53 PM
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Raquel Jovani-Abril, Mathieu Gibilaro, Arne Janssen, Rachel Eloirdi, Joseph Somers, et al.. Synthesis of nc-UO 2 by controlled precipitation in aqueous phase. Journal of Nuclear Materials, Elsevier, 2016, 477, pp.298-304. ⟨10.1016/j.jnucmat.2016.04.050⟩. ⟨hal-02134867⟩

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