In vitro production of DNA minicircles less than 250 base pairs

Jean-Marc Malinge (1) , Patrick Midoux (1) , Thomas Thibault (1) , Chantal Pichon (1)

1 CBM - Centre de biophysique moléculaire

A method for the in vitro production of DNA minicircles comprising steps of: a) providing nicked double- stranded oligodeoxynucleotides bunt- ended substrates having at least one phosphorylated 5’ end, b) performing a ligase-mediated circularization on a reactionmixture comprising the said nicked double-stranded oligodeoxynucleotides substrates and a DNA bending protein, and c) obtaining DNA minicircles.

Domaines: Sciences du Vivant [q-bio]

Jean-Marc Malinge, Patrick Midoux, Thomas Thibault, Chantal Pichon. In vitro production of DNA minicircles less than 250 base pairs. France, Patent n° : EP2735614A1. 2014. ⟨hal-02134791⟩