In vitro production of DNA minicircles less than 250 base pairs

Abstract : A method for the in vitro production of DNA minicircles comprising steps of: a) providing nicked double- stranded oligodeoxynucleotides bunt- ended substrates having at least one phosphorylated 5’ end, b) performing a ligase-mediated circularization on a reactionmixture comprising the said nicked double-stranded oligodeoxynucleotides substrates and a DNA bending protein, and c) obtaining DNA minicircles.
Document type :
Patents
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-02134791
Contributor : Laëtitia Legoupil <>
Submitted on : Monday, May 20, 2019 - 5:18:03 PM
Last modification on : Thursday, June 20, 2019 - 12:22:09 PM

Identifiers

  • HAL Id : hal-02134791, version 1

Collections

Citation

Jean-Marc Malinge, Patrick Midoux, Thomas Thibault, Chantal Pichon. In vitro production of DNA minicircles less than 250 base pairs. France, Patent n° : EP2735614A1. 2014. ⟨hal-02134791⟩

Share

Metrics

Record views

14