Immunological end spectroscopic studies of poly(dG-dC).poly(dG-dC) modified by cis-diamminedicholoroplatinum(II) - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 1984

Immunological end spectroscopic studies of poly(dG-dC).poly(dG-dC) modified by cis-diamminedicholoroplatinum(II)

Résumé

The conformational changes induced by the binding of cis-diamminedichloroplatinum(II) to poly(dG-dC).poly(dG-dC) have been studied by reaction with specific antibodies, by circular dichroism and 31P nuclear magnetic resonance. Polyclonal and monoclonal antibodies to Z-DNA bind to platinated poly(dG-dC).poly(dG-dC) at low and high ionic strength. Antibodies elicited in rabbits immunized with the platinated polynucleotide bind to double stranded polynucleotides known to adopt the Z-conformation. At low and high ionic strength the circular dichroism spectrum of platinated poly(dG-dC).poly(dG- dC) does not resemble that of poly(dG-dC).poly(dG-dC) (B or Z conformation). At low ionic strength, the characteristic 31P nuclear magnetic resonance spectrum of the Z-form is not detected. It appears only at high ionic strength, as a component of a more complex spectrum.

Dates et versions

hal-02134761 , version 1 (20-05-2019)

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Citer

Jean-Marc Malinge, Marius Ptak, Marc Leng. Immunological end spectroscopic studies of poly(dG-dC).poly(dG-dC) modified by cis-diamminedicholoroplatinum(II). Nucleic Acids Research, 1984, 12 (14), pp.5767-5778. ⟨10.1093/nar/12.14.5767⟩. ⟨hal-02134761⟩
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