Importance of DNA conformation in the reaction with cis-dichlorodiammine platinum (II) - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Biosciences Année : 1985

Importance of DNA conformation in the reaction with cis-dichlorodiammine platinum (II)

Résumé

Cis-dichlorodiammine platinum (II) has been reacted with synthetic polynucleotides either in B or in Z conformation. The binding of cis-dichlorodiammine platinum (II) stabilizes the Z conformation when reacted with poly (dG-m5dC) ·poly (dG-m5dC) in the Z conformation as shown by circular dichroism and by the antibodies to Z-DNA. On the other hand, the binding of cis-dichlorodiammine platinum (II) stabilizes a new conformation when reacted with poly(dG-dC)·poly(dG-dC) or poly (dG-m5dC)·poly(dG-m5dC) in the B conformation. The antibodies to Z-DNA bind to these platinated polynucleotides. In rabbits, the injection of platinated poly (dG-dC) poly (dG-dC) induces the synthesis of antibodies which recognize Z-DNA. In low salt conditions, the circular dichroism spectra of these platinated polynucleotides differ from those of B-DNA or Z-DNA. The characteristic31P nuclear magnetic resonance spectrum of Z-DNA is not detected. It appears only at high ionic strength, as a component of a more complex spectrum.

Dates et versions

hal-02134748 , version 1 (20-05-2019)

Identifiants

Citer

Marc Leng, Jean-Marc Malinge, Marius Ptak. Importance of DNA conformation in the reaction with cis-dichlorodiammine platinum (II). Journal of Biosciences, 1985, 8 (3), pp.721-729. ⟨10.1007/bf02702770⟩. ⟨hal-02134748⟩
25 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More