Leukemic CD3+ LGL share functional properties with their CD8+ CD57+ cell counterpart expanded after BMT.

Abstract : Leukemic T-LGL (large granular lymphocyte) composed of clonal CD3+ TCR alphabeta+ CD8+ CD57+ cells were compared with oligoclonally CD3+ CD8hi+ CD57- lymphocytes expanded after BMT. Leukemic CD3+ CD8hi+ CD57+ LGL showed several phenotypic differences such as an upregulation of CD16 and adhesion molecules (mainly CD11c, CD58 and CD54), activation markers and an exclusive CD45RA isoform expression. Unstimulated CD3+ CD8+ CD57+ LGL from both leukemic and BMT donors spontaneously developed an ex vivo CTL-like CD3-redirected cytotoxicity but no NK cell activity. Different stimuli (PHA, PMA or rhIL-2) induced similar cytotoxic profiles after a 6-day culture involving a CD3-redirected lysis predominating over a low NK cell activity. However, culture of leukemic LGL with these stimuli allowed either a 2 week persistence (PMA or rhIL-2) of CD8+ CD57+ LGL or their disappearance after 3 days (PHA). Furthermore, leukemic CD8hi+ CD57+ T lymphocytes produced an inhibitor of cytotoxic functions as previously described for BMT recipients' CD8+ CD57+ cells. Thus, despite some phenotypic differences between both cell sources, leukemic CD57+ T-LGL display the same functional characteristics of cytotoxic effector and immunoregulatory T cells as CD8+ CD57+ T cells from BMT recipients which might represent their normal counterpart.
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https://hal.archives-ouvertes.fr/hal-02129951
Contributor : Laëtitia Legoupil <>
Submitted on : Wednesday, May 15, 2019 - 2:24:40 PM
Last modification on : Thursday, May 16, 2019 - 1:40:37 AM

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  • HAL Id : hal-02129951, version 1
  • PUBMED : 10025897

Citation

Lucile Mollet, B. Fautrel, V. Leblond, F. Bergeron, H Merle-Béral, et al.. Leukemic CD3+ LGL share functional properties with their CD8+ CD57+ cell counterpart expanded after BMT.. Leukemia, Nature Publishing Group: Open Access Hybrid Model Option B, 1999, 13 (2), pp.230-40. ⟨hal-02129951⟩

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