The Size of the Topological Domain Modulates the B-Z Transition of a (TG) n Containing Repeat

Abstract : Under negative superhelical stress, long (TG)n containing repeats experience a stepwise multiple B-Z transitions. We have investigated the effect of the plasmid size on this transitional behavior. A 66-bp (TG)n containing repeat from the 5'-untranscribed region of mouse ribosomal DNA was inserted in a 3-kb, a 6.5-kb and a 12.5-kb plasmids and its supercoil-driven B-Z transition was followed by OsO4 probing of topoisomer-populations. Our results show a clear correlation between the size of the topological domain and the extent of the region that converts cooperatively into Z-DNA at the initial transition.
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Annie-Claude Albert, Marc Leng, A. Rachid Rahmouni. The Size of the Topological Domain Modulates the B-Z Transition of a (TG) n Containing Repeat. Journal of Biomolecular Structure and Dynamics, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 1995, 13 (1), pp.47-56. ⟨10.1080/07391102.1995.10508820⟩. ⟨hal-02127217⟩

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