Gradual and oriented B-Z transition in 5'-untranscribed region of mouse ribosomal DNA.

Abstract : The structural transition of an alternating purine-pyrimidine sequence (CG)5(TG)28) from the 5'-untranscribed region of the mouse ribosomal DNA was analyzed by two-dimensional gel electrophoresis and chemical probes. The repeat undergoes a supercoil-dependent gradual and oriented B-Z transition. At a threshold level of negative supercoiling, a limited region of the repeat encompassing the (CG)5 motif converts cooperatively into Z-DNA. As the superhelical stress increases, the Z-structure propagates along the remaining part of the repeat by successive transitions until the full-length sequence is converted. By in situ OsO4 probing experiments, we show also that this (TG)n-containing repeat adopts the Z-structure in Escherichia coli.
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https://hal.archives-ouvertes.fr/hal-02127210
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Submitted on : Monday, May 13, 2019 - 11:57:19 AM
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  • HAL Id : hal-02127210, version 1
  • PUBMED : 8034685

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A.C Albert, A. Roman, G. Bouche, M. Leng, A. Rachid Rahmouni. Gradual and oriented B-Z transition in 5'-untranscribed region of mouse ribosomal DNA.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1994, 269 (30), pp.19238-44. ⟨hal-02127210⟩

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