Reaction of nucleic acids with cis-diamminedichloroplatinum(II): interstrand cross-links.

Abstract : In the reaction of cis-diamminedichloroplatinum(II) (cis-DDP) with double-helical (dC-dG)4.(dC-dG)4 or (dC-dG)5.(dC-dG)5, intrastrand and interstrand cross-links between two guanine residues are formed. This is shown by gel electrophoresis in denaturing conditions of the reaction products and by high-performance liquid chromatography (HPLC) analysis of the products digested with nuclease P1. In the reaction of cis-DDP and poly(dG-dC).poly(dG-dC), at relatively low levels of platination, it is mainly interstrand cross-links between two guanine residues that are formed. This is shown by HPLC analysis of the nuclease P1 digest and by gel electrophoresis in denaturing and nondenaturing conditions of the platinated polymer after cleavage with the restriction enzyme HhaI. Moreover, the antibodies to platinated poly(dG-dC).poly(dG-dC) cross-react with the interstrand cross-linked (dC-dG)4 or (dC-dG)5 but not with the intrastrand cross-linked (dC-dG)4 or (dC-dG)5. These antibodies cross-react with platinated natural DNA. The amount of interstrand cross-links deduced from radioimmunoassays (0.5% of the total bound platinum) is lower than that (2%) deduced by gel electrophoresis in denaturing conditions of a platinated DNA restriction fragment. By gel electrophoresis, it is also shown that in vitro the isomer trans-DDP is more efficient in forming interstrand cross-links than cis-DDP.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-02127140
Contributor : Laëtitia Legoupil <>
Submitted on : Monday, May 13, 2019 - 11:29:39 AM
Last modification on : Tuesday, May 14, 2019 - 1:25:07 AM

Identifiers

  • HAL Id : hal-02127140, version 1
  • PUBMED : 3427071

Collections

Citation

A. Rachid Rahmouni, M. Leng. Reaction of nucleic acids with cis-diamminedichloroplatinum(II): interstrand cross-links.. Biochemistry, American Chemical Society, 1987, 26 (23), pp.7229-34. ⟨hal-02127140⟩

Share

Metrics

Record views

3