DPPC Bilayers in Solutions of High Sucrose Content

Mattia Morandi (1) , Mathieu Sommer (1) , Monika Kluzek (1) , Fabrice Thalmann (1) , André P. Schroder (1) , Carlos M. Marques (1)

1 ICS - Institut Charles Sadron

The properties of lipid bilayers in sucrose solutions have been intensely scrutinized over recent decades because of the importance of sugars in the field of biopreservation. However, a consensus has not yet been formed on the mechanisms of sugar-lipid interaction. Here, we present a study on the effect of sucrose on 1,2-dipalmitoyl-sn-glycero-3-phos-phocholine bilayers that combines calorimetry, spectral fluorimetry, and optical microscopy. Intriguingly, our results show a significant decrease in the transition enthalpy but only a minor shift in the transition temperature. Our observations can be quantitatively accounted for by a thermodynamic model that assumes partial delayed melting induced by sucrose adsorption at the membrane interface.

Domaines: Matière Molle [cond-mat.soft]

Mattia Morandi, Mathieu Sommer, Monika Kluzek, Fabrice Thalmann, André P. Schroder, et al.. DPPC Bilayers in Solutions of High Sucrose Content. Biophysical Journal, 2018, 114 (9), pp.2165-2173. ⟨10.1016/j.bpj.2018.04.003⟩. ⟨hal-02126590⟩