Converting any Faradaic Current Generated at an Electrode under Potentiostatic Control into a Remote Fluorescence Signal

Abstract : We describe the development of an original faradaic current to fluorescence conversion scheme. The proposed instrumental strategy consists in coupling the electrochemical reaction of any species at an electrode under potentiostatic control, with the fluorescence emission of a species produced at the counter electrode. In order to experimentally validate this scheme, the fluorogenic species resazurin is chosen as a fluorescent reporter molecule, and its complex reduction mechanism is first studied in unprecedented details. This kinetic study is carried out by recording simultaneous cyclic voltammograms and voltfluorograms at the same electrode. Numerical simulations are used to account for the experimental current and fluorescence signals, to analyze their degree of correlation, and to decipher their relation to resazurin reduction kinetics. It is then shown that, provided that the reduction of resazurin takes place at a micrometer-sized electrode, the fluorescence emission perfectly tracks the faradaic current. By implementing this ideal configuration at the counter electrode of a potentiostatic setup, it is finally demonstrated that the oxidation reaction of a non-fluorescent species at the working electrode can be quantitatively transduced into simultaneous emission of fluorescence.
Document type :
Journal articles
Complete list of metadatas

Cited literature [20 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02120999
Contributor : Christophe Demaille <>
Submitted on : Monday, May 6, 2019 - 12:48:18 PM
Last modification on : Sunday, May 12, 2019 - 1:14:30 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : 2019-10-29

Please log in to resquest access to the document

Identifiers

Citation

Rabia Djoumer, Agnès Anne, Arnaud Chovin, Christophe Demaille, Corinne Dejous, et al.. Converting any Faradaic Current Generated at an Electrode under Potentiostatic Control into a Remote Fluorescence Signal. Analytical Chemistry, American Chemical Society, In press, ⟨10.1021/acs.analchem.9b00851⟩. ⟨hal-02120999⟩

Share

Metrics

Record views

24