Detection of minor adducts in cisplatin-modified DNA by transcription footprinting.

Abstract : Two DNA restriction fragments containing either a d(GC)5 or a d(TTGCTTGATTAGTTGTGTT) insert were subjected to reaction with cis-diamminedichloroplatinum(II) and were then used as templates for RNA synthesis by T7 RNA polymerase. Within the d(GC)5 insert, interstrand cross-links are preferentially formed. Within the second insert, the reactivity order of the potential binding sites is d(ApG) > d(GpC/GpC) = d(GpA) > d(GpTpG). In the presence of cyanide ions, the adducts are much less stable at the d(GpA) sites than at the d(GpCpG) sites, in double-stranded DNA.
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https://hal.archives-ouvertes.fr/hal-02119335
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Submitted on : Friday, May 3, 2019 - 4:56:20 PM
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  • HAL Id : hal-02119335, version 1
  • PUBMED : 8495748

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M. Decoville, A. Schwartz, D. Locker, M. Leng. Detection of minor adducts in cisplatin-modified DNA by transcription footprinting.. FEBS Letters, Wiley, 1993, 323 (1-2), pp.55-8. ⟨hal-02119335⟩

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