Deletions induced in the white and vermilion genes of Drosophila melanogaster by the antitumor drug cis-dichlorodiammineplatinum(II).

J Cizeau , Martine Decoville , M. Leng , D. Locker (1)

1 CBM - Centre de biophysique moléculaire

This paper describes the analysis of cisplatin induced mutations at the white (w) and vermilion (v) loci located on the X chromosome of Drosophila melanogaster. Twenty-eight w and eight v mutants have been found in a male genetic context and 42 w mutants in a female genetic context. In these latter experiments, genetic analysis showed the presence of multi-locus deficiencies in 18 out of 42 w mutants. Eighteen w and three v intragenic mutations were analyzed at the molecular level. Seventeen w and three v mutants carry deletions within the gene, ranging in size from 4 to 109 base pairs. Sequence analysis of the mutants indicates that most of them were produced by non-homologous recombinational events occurring between short (2-5 bp) sequence repeats on both sides of the deletion, one repeat being retained at the new junction. These results differ largely from those obtained in prokaryotic and other eukaryotic cells.

Domaines: Sciences du Vivant [q-bio]

J Cizeau, Martine Decoville, M. Leng, D. Locker. Deletions induced in the white and vermilion genes of Drosophila melanogaster by the antitumor drug cis-dichlorodiammineplatinum(II).. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1994, 311 (1), pp.31-8. ⟨hal-02119331⟩