Voltammetric Sensor Based on a Double-Layered Molecularly Imprinted Polymer for Testosterone

Hana Fourou 1, 2 Mohamed Braiek 3 Anne Bonhommé 4 Florence Lagarde 5 Ali Zazoua 2 Nicole Jaffrezic-Renault 3, *
* Corresponding author
3 Interfaces & biosensors - Interfaces & biocapteurs
ISA - Institut des Sciences Analytiques
4 spectroscopies vibrationnelles
ISA - Institut des Sciences Analytiques
5 SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes
ISA - Institut des Sciences Analytiques
Abstract : A novel strategy to improve the sensitivity of molecularly imprinted polymer sensors based on a double-layered molecularly imprinted polymer hybrid film-modified gold electrode was investigated for the determination of testosterone. A conductive poly(anilinomethyltriethoxysilane) film was electrodeposited on the surface of the electrode, and a molecularly imprinted polysiloxane thin film was formed on the conductive layer by a sol-gel process to enhance the electrochemical response. The performance of the sensor for testosterone was investigated by square-wave voltammetry in the presence of a hexacyanoferrate/ferrite as a redox probe. The prepared sensor provided high recognition for testosterone with a broad linear range from 10 to 100fM and a low detection limit of 10fM. Good sensitivity, selectivity, and stability were observed with relative standard deviations less than 5%. Good recovery of testosterone was obtained when the sensor was used for the determination of testosterone in fortified urine.
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Journal articles
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https://hal.archives-ouvertes.fr/hal-02116237
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Submitted on : Tuesday, April 30, 2019 - 5:55:33 PM
Last modification on : Wednesday, May 1, 2019 - 1:20:12 AM

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Hana Fourou, Mohamed Braiek, Anne Bonhommé, Florence Lagarde, Ali Zazoua, et al.. Voltammetric Sensor Based on a Double-Layered Molecularly Imprinted Polymer for Testosterone. Analytical Letters, Taylor & Francis, 2017, 51 (3), pp.312-322. ⟨10.1080/00032719.2017.1298118⟩. ⟨hal-02116237⟩

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