Voltammetric glucose biosensor based on glucose oxidase encapsulation in a chitosan-kappa-carrageenan polyelectrolyte complex

Abstract : In this work, a new design of voltanunetric glucose biosensor, based on the encapsulation of glucose oxidase (GOx) in a chitosan/kappa-carrageenan (CHIT/CAR) polyelectrolyte complex (PEC) using a simple coacervation process is presented. A conductometric monitoring of this is performed. Spectroscopic and morphological characterization of the PEC film encapsulating GOx is carried out. Compared to biosensors based on a chitosan film, a more sensitive voltammetric detection of glucose is obtained. Using square wave voltammetry (SWV), the CHIT/CAR PEC based biosensor exhibits a wide linearity range from 5 mu M to 7 mM glucose with a detection limit of 5 mu M. Excellent selectivity against ascorbic acid, uric acid and urea is observed and the applicability of the biosensor for glucose detection in spiked saliva samples was demonstrated.
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https://hal.archives-ouvertes.fr/hal-02115795
Contributor : Agnès Bussy <>
Submitted on : Tuesday, April 30, 2019 - 2:55:04 PM
Last modification on : Tuesday, June 25, 2019 - 5:35:42 AM

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Ilhem Rassas, Mohamed Braiek, Anne Bonhommé, François Bessueille, Guy Raffin, et al.. Voltammetric glucose biosensor based on glucose oxidase encapsulation in a chitosan-kappa-carrageenan polyelectrolyte complex. Materials Science and Engineering: C, Elsevier, 2019, 95, pp.152-159. ⟨10.1016/j.msec.2018.10.078⟩. ⟨hal-02115795⟩

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