Involvement of thymosin beta 4 and endoproteinase Asp-N in the biosynthesis of the tetrapeptide AcSerAspLysPro a regulator of the hematopoietic system.

Abstract : It is shown that AcSDKP a new regulator of the hematopoietic system can be generated from thymosin beta 4 by a one-step enzymatic cleavage in vitro and in vivo. AcSDKP and T beta 4 were both detected in bone marrow cells (BMC). Incubation of [3H]T beta 4 with either intact or lysed BMC led to the formation of [3H]AcSDKP whereas the labelled tetrapeptide was not degraded under these conditions. Model enzymatic degradation of T beta 4 carried out with bacterial enzymes suggests that a mammalian endoproteinase Asp-N might be involved in the formation of AcSDKP through the specific cleavage of the 4Pro-5 Asp peptide bond of T beta 4.
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https://hal.archives-ouvertes.fr/hal-02114986
Contributor : Laëtitia Legoupil <>
Submitted on : Tuesday, April 30, 2019 - 9:11:28 AM
Last modification on : Wednesday, May 1, 2019 - 1:20:16 AM

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  • HAL Id : hal-02114986, version 1
  • PUBMED : 2253778

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Catherine Grillon, K Rieger, J. Bakala, D Schott, M Morgat, et al.. Involvement of thymosin beta 4 and endoproteinase Asp-N in the biosynthesis of the tetrapeptide AcSerAspLysPro a regulator of the hematopoietic system.. FEBS Letters, Wiley, 1990, 274 (1-2), pp.30-4. ⟨hal-02114986⟩

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