Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells

Marjorie Monleau , Sophie Bonnel (1) , Thierry Gostan (2) , Dominique Blanchard (3) , Valérie Courgnaud (4) , Charles-Henri Lecellier (2)

1 I2MC - Institut des Maladies Métaboliques et Cardiovasculaires
2 IGMM - Institut de Génétique Moléculaire de Montpellier
3 LMRS - Laboratoire de Mathématiques Raphaël Salem
4 TransVIHMI - Recherches Translationnelles sur le VIH et les maladies infectieuses endémiques et émergentes

microRNAs (miRNAs) play crucial roles in major biological processes and their deregulations are often associated with human malignancies. As such, they represent appealing candidates as targets of innovative therapies. Another interesting aspect of their biology is that they are present in various biological fluids where, advantageously, they appear to be very stable. A plethora of studies have now reported their potential as biomarkers that can be used in diagnosis, prognosis and/or theranostic issues. However, the application of circulating miRNAs in clinical practices still requires the identification of highly efficient, robust and reproducible methods for their isolation from biological samples.In that context, we performed an independent cross-comparison of three commercially available RNA extraction kits for miRNAs isolation from human blood samples (Qiagen and Norgen kits as well as the new NucleoSpin miRNAs Plasma kit from Macherey-Nagel). miRNAs were further profiled using the Taqman Low Density Array technology.

Domaines: Génomique, Transcriptomique et Protéomique [q-bio.GN]

Marjorie Monleau, Sophie Bonnel, Thierry Gostan, Dominique Blanchard, Valérie Courgnaud, et al.. Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells. BMC Genomics, 2014, 15 (1), pp.395. ⟨10.1186/1471-2164-15-395⟩. ⟨hal-02106442⟩