L. Bataille, W. Dieryck, A. Hocquellet, C. Cabanne, K. Bathany et al., Recombinant production and purification of short hydrophobic Elastin-like polypeptides with low transition temperatures, 2016.
URL : https://hal.archives-ouvertes.fr/hal-01360540

. Purif, , vol.121, pp.81-87

L. Bataille, W. Dieryck, A. Hocquellet, C. Cabanne, K. Bathany et al., Expression and purification of short hydrophobic elastin-like polypeptides with maltose-binding protein as a solubility tag, 2015.
URL : https://hal.archives-ouvertes.fr/hal-01372375

. Purif, , vol.110, pp.165-171

A. Ben-bassat, K. Bauer, S. Y. Chang, K. Myambo, A. Boosman et al., Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure, J. Bacteriol, vol.169, pp.751-758, 1987.

M. Biancalana and S. Koide, Molecular mechanism of Thioflavin-T binding to amyloid fibrils, Biochim. Biophys. Acta, vol.1804, pp.1405-1417, 2010.

,

, Journal of Biotechnology, vol.298, pp.35-44, 2019.

S. E. Bondos and A. Bicknell, Detection and prevention of protein aggregation before, during, and after purification, Anal. Biochem, vol.316, pp.223-231, 2003.

, , pp.59-68

K. Changi, B. Bosnjak, C. Gonzalez-obeso, R. Kluger, J. C. Rodríguez-cabello et al., Biocompatibility and immunogenicity of elastinlike recombinamer biomaterials in mouse models, J. Biomed. Mater. Res. Part A, vol.106, pp.924-934, 2018.

T. Christensen, W. Hassouneh, K. Trabbic-carlson, and A. Chilkoti, Predicting transition temperatures of elastin-like polypeptide fusion proteins, Biomacromolecules, vol.14, pp.1514-1519, 2013.

J. L. Drury and D. J. Mooney, Hydrogels for tissue engineering: scaffold design variables and applications, Biomaterials, vol.24, issue.03, pp.340-345, 2003.

T. Fukuda, S. Takeda, R. Xu, H. Ochi, S. Sunamura et al., Sema3A regulates bonemass accrual through sensory innervations, Nature, vol.497, pp.490-493, 2013.

,

D. S. Grant, J. L. Kinsella, R. Fridman, R. Auerbach, B. A. Piasecki et al., Interaction of Endothelial-Cells With a Laminin-a Chain Peptide (Sikvav) Invitro and Induction of Angiogenic Behavior Invivo, J. Cell. Physiol, vol.153, pp.614-625, 1992.

M. Grellier, L. Bordenave, and J. Amédée, Cell-to-cell communication between, Journal of Biotechnology, vol.298, pp.35-44, 2009.

, implications for tissue engineering, Trends Biotechnol, vol.27, pp.562-71

J. Guerrero, S. Catros, S. Derkaoui, C. Lalande, R. Siadous et al., Cell interactions between human progenitor-derived endothelial cells and human mesenchymal stem cells in a three-dimensional macroporous polysaccharide-based scaffold promote osteogenesis, Acta Biomater, vol.9, pp.8200-8213, 2013.

,

P. H. Hirel, M. J. Schmitter, P. Dessen, G. Fayat, and S. Blanquet, Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid, Proc. Natl. Acad. Sci. U. S. A, vol.86, pp.8247-51, 1989.

H. Hosseinkhani, Y. Hiraoka, C. H. Li, Y. R. Chen, D. S. Yu et al., Engineering three-dimensional collagen-IKVAV matrix to mimic neural microenvironment, ACS Chem. Neurosci, vol.4, pp.1229-1235, 2013.

,

A. Ibáñez-fonseca, T. L. Ramos, I. González-de-torre, L. I. Sánchez-abarca, S. Muntión et al., Biocompatibility of two model elastin-like recombinamer-based hydrogels formed through physical or chemical cross-linking for various applications in tissue engineering and regenerative medicine, J. Tissue Eng. Regen. Med, vol.12, pp.1450-1460, 2018.

,

K. B. Jones, A. V. Mollano, J. Morcuende, R. R. Cooper, and C. L. Saltzman, Bone and brain: a review of neural, hormonal, and musculoskeletal connections, Iowa Orthop. J. Journal of Biotechnology, vol.298, pp.35-44, 2004.

R. Kaneko and N. Kitabatake, Heat-Induced Formation of Intermolecular Disulfide Linkages between Thaumatin Molecules That Do Not Contain Cysteine Residues, 1999.

. Agric, Food Chem, vol.47, pp.4950-4955

R. Khurana, C. Coleman, C. Ionescu-zanetti, S. A. Carter, V. Krishna et al., Mechanism of thioflavin T binding to amyloid fibrils, J. Struct. Biol, vol.151, pp.229-238, 2005.

H. Kim, W. Caspar, T. Shah, S. B. Hsieh, and A. H. , Effects of proinflammatory cytokines on axonal outgrowth from adult rat lumbar dorsal root ganglia using a novel threedimensional culture system, Spine J, vol.15, pp.1823-1831, 2015.

,

K. Trabbic-carlson, L. A. Setton, *. Chilkoti, and A. , Swelling and Mechanical Behaviors of Chemically Cross-Linked Hydrogels of Elastin-like Polypeptides, 2003.

D. Koch, W. J. Rosoff, J. Jiang, H. M. Geller, and J. S. Urbach, Strength in the periphery: growth cone biomechanics and substrate rigidity response in peripheral and central nervous system neurons, Biophys. J, vol.102, pp.452-60, 2012.

,

J. R. Kramer, R. Petitdemange, L. Bataille, K. Bathany, A. L. Wirotius et al., Quantitative Side-Chain Modifications of Methionine-Containing Elastin-Like Polypeptides as a Versatile Tool to Tune Their Properties, vol.4, pp.1283-1286, 2015.
URL : https://hal.archives-ouvertes.fr/hal-01361956

,

, Journal of Biotechnology, vol.298, pp.35-44, 2019.

Y. Lee, H. J. Kim, C. K. Park, W. S. Kim, Z. H. Lee et al., Novel extraneural role of neurite outgrowth inhibitor A: Modulation of osteoclastogenesis via positive feedback regulation of nuclear factor of activated T cell cytoplasmic 1, J. Bone Miner. Res, vol.27, pp.1043-1054, 2012.

B. Li, D. O. Alonso, and V. Daggett, The molecular basis for the inverse temperature transition of elastin, J. Mol. Biol, vol.305, pp.581-92, 2001.

B. Li, T. Qiu, P. Zhang, X. Wang, Y. Yin et al., IKVAV regulates ERK1/2 and Akt signalling pathways in BMMSC population growth and proliferation, Cell Prolif, vol.47, pp.133-145, 2014.

S. Madduri and B. Gander, Schwann cell delivery of neurotrophic factors for peripheral nerve regeneration, J. Peripher. Nerv. Syst, vol.15, pp.93-103, 2010.

S. A. Malin, B. M. Davis, and D. C. Molliver, Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity, 2007.

, Nat. Protoc, vol.2, pp.152-160

D. E. Meyer, Purification of recombinant proteins by fusion with thermally-responsive polypeptides, Nat. Biotechnol, vol.17, pp.1112-1115, 1999.

,

D. E. Meyer and A. Chilkoti, Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides, Biomacromolecules, vol.5, pp.846-851, 2004.

M. Miao, J. T. Cirulis, S. Lee, and F. W. Keeley, Structural determinants of cross-linking and hydrophobic domains for self-assembly of elastin-like polypeptides, Biochemistry Journal of Biotechnology, vol.298, pp.35-44, 2005.

, , vol.44, pp.14367-75

M. Nakamura, M. Mie, H. Mihara, M. Nakamura, and E. Kobatake, Construction of multi-functional extracellular matrix proteins that promote tube formation of endothelial cells, Biomaterials, vol.29, pp.2977-2986, 2008.

D. L. Nettles, A. Chilkoti, and L. A. Setton, Applications of elastin-like polypeptides in tissue engineering, Adv. Drug Deliv. Rev, vol.62, pp.1479-1485, 2010.

,

D. L. Nettles, K. Kitaoka, N. A. Hanson, C. M. Flahiff, B. A. Mata et al., In situ crosslinking elastin-like polypeptide gels for application to articular cartilage repair in a goat osteochondral defect model, Tissue Eng. Part A, vol.14, pp.1133-1173, 2008.

. Nicol, D. C. Gowda, and D. W. Urry, Cell adhesion and growth on synthetic elastomeric matrices containing Arg-Gly-Asp-Ser-3, J. Biomed. Mater. Res, vol.26, pp.393-413, 1992.

,

K. L. Niece, C. Czeisler, V. Sahni, V. Tysseling-mattiace, E. T. Pashuck et al., Modification of gelation kinetics in bioactive peptide amphiphiles, Biomaterials, vol.29, pp.4501-4510, 2008.

M. Nomizu, B. S. Weeks, C. A. Weston, W. H. Kim, H. K. Kleinman et al., Structure-activity study of a laminin ?1 chain active peptide segment Ile, 1995.

. Val, FEBS Lett, vol.365, p.475

G. Pasut and F. M. Veronese, Polymer-drug conjugation, recent achievements and general strategies, Prog. Polym. Sci, vol.32, pp.933-961, 2007.

, Journal of Biotechnology, vol.298, pp.35-44, 2019.

,

S. Prieto, A. Shkilnyy, C. Rumplasch, A. Ribeiro, F. J. Arias et al., Biomimetic calcium phosphate mineralization with multifunctional elastin-like recombinamers, Biomacromolecules, vol.12, pp.1480-1486, 2011.

,

L. Y. Santiago, R. W. Nowak, J. Peter-rubin, and K. G. Marra, Peptide-surface modification of poly(caprolactone) with laminin-derived sequences for adipose-derived stem cell applications, Biomaterials, vol.27, pp.2962-2971, 2006.

,

A. Shekaran and A. J. Garcia, Nanoscale engineering of extracellular matrix-mimetic bioadhesive surfaces and implants for tissue engineering, Biochim. Biophys. Acta, vol.1810, pp.350-60, 2011.

D. I. Silva, B. P. Santos, . Dos, J. Leng, H. Oliveira et al., Dorsal root ganglion neurons regulate the transcriptional and translational programs of osteoblast differentiation in a microfluidic platform, Cell Death Dis, vol.8, 2017.

G. A. Silva, C. Czeisler, K. L. Niece, E. Beniash, D. A. Harrington et al., Selective Differentiation of Neural Progenitor Cells by High -Epitope Density Nanofibers. Science, vol.303, pp.1352-1355, 2004.

,

A. M. Testera, A. Girotti, I. G. De-torre, L. Quintanilla, M. Santos et al., Biocompatible elastin-like click gels: design, synthesis and characterization, J. Mater. Sci. Mater. Med, vol.26, pp.35-44, 2015.

K. Trabbic-carlson, L. A. Setton, and A. Chilkoti, Swelling and Mechanical Behaviors of Chemically Cross-Linked Hydrogels of Elastin-like Polypeptides Swelling and Mechanical Behaviors of Chemically Cross-Linked Hydrogels of Elastin-like Polypeptides, Society, 2003.

D. W. Urry, Free energy transduction in polypeptides and proteins based on inverse temperature transitions, Prog. Biophys. Mol. Biol, vol.57, pp.23-57, 1992.

,

D. W. Urry, T. L. Trapane, and K. U. Prasad, Phase-structure transitions of the elastin polypentapeptide-water system within the framework of composition-temperature studies, Biopolymers, vol.24, pp.2345-2356, 1985.

H. Wang, L. Cai, A. Paul, A. Enejder, and S. C. Heilshorn, Hybrid elastin-like polypeptide-polyethylene glycol (ELP-PEG) hydrogels with improved transparency and independent control of matrix mechanics and cell ligand density, Biomacromolecules, vol.15, pp.3421-3429, 2014.

A. Wilkins, K. Kemp, M. Ginty, K. Hares, E. Mallam et al., Human bone marrow-derived mesenchymal stem cells secrete brain-derived neurotrophic factor which promotes neuronal survival in vitro, Stem Cell Res, vol.3, pp.63-70, 2009.

,

D. Xu, D. Asai, A. Chilkoti, and S. L. Craig, Rheological Properties of CysteineContaining Elastin-Like Polypeptide Solutions and Hydrogels, with or without ?-mercaptoethanol (0.68 M final concentration). After heating for 5 min at, vol.13, p.95, 2012.

, InstantBlue TM was used to stain the gel. The two forms of dimers probably correspond to dimers formed, °C, samples were loaded onto the gel. M: Precision Plus Protein Standards

, Journal of Biotechnology, vol.298, pp.35-44, 2019.