Monitoring of Legionella pneumophila viability after chlorine dioxide treatment using flow cytometry

Abstract : The viability of three Legionella pneumophila strains was monitored after chlorine dioxide (ClO2) treatment using a flow cytometric assay. Suspensions of L. pneumophila cells were submitted to increasing concentrations of ClO2. Culturable cells were still detected when using 4 mg/L, but could no longer be detected after exposure to 6 mg/L of ClO2, although viable but not culturable (VBNC) cells were found after exposure to 4–5 mg/L of ClO2. When testing whether these VBNC were infective, two of the strains were resuscitated after co-culture with Acanthamoeba polyphaga, but neither of them could infect macrophage-like cells.
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Submitted on : Monday, April 15, 2019 - 5:12:08 PM
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Pascale Mustapha, Thibaut Epalle, Séverine Allegra, Françoise Girardot, Olivier Garraud, et al.. Monitoring of Legionella pneumophila viability after chlorine dioxide treatment using flow cytometry. Research in Microbiology, Elsevier, 2015, 166 (3), pp.215-219. ⟨10.1016/j.resmic.2015.01.004⟩. ⟨hal-02100309⟩

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