Displacement fields using correlation methods as a tool to investigate cell migration in 3D collagen gels

Arnold Fertin 1, * Laure Laforgue 2 Alain Duperray 3 Valérie Laurent Yves Usson 1, * Claude Verdier 4, *
* Corresponding author
1 TIMC-IMAG-DyCTiM - Dynamique Cellulaire et Tissulaire- Interdisciplinarité, Modèles & Microscopies
TIMC-IMAG - Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525
2 LIPHY-DYFCOM
LIPhy - Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères]
4 DYFCOM - DYnamique des Fluides COmplexes et Morphogénèse [Grenoble]
LIPhy - Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] : LIPhy
Abstract : Living cells embedded in a complex Extra–Cellular Matrix (ECM) migrate in a sophisticated way thanks to adhesions to matrix fibres and contractility. It is important to know what kind of forces are exerted by the cells. Here we use reflectance confocal microscopy to locate fibres accurately and determine displacement fields. Correlation techniques are used to this aim, coupled with proper digital image processing. Benchmark tests validate the method in the case of shear and stretching motions. Finally, the method is tested successfully for studying cancer cells migrating in collagen gels of different concentration. KEYWORDS: Displacement field, phase correlation, confocal microscopy, cell migration, fibres, cancer cells.
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Submitted on : Monday, June 24, 2019 - 6:21:23 PM
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Arnold Fertin, Laure Laforgue, Alain Duperray, Valérie Laurent, Yves Usson, et al.. Displacement fields using correlation methods as a tool to investigate cell migration in 3D collagen gels. 2019. ⟨hal-02086505v2⟩

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