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, Each transcript is represented once and each 925 line represents one transcript. Colors: yellow, over-represented transcripts; purple, under-926 represented transcripts; and black, unchanged relative to levels in control naïve snails. 927 928 S2 Fig: Mortality of B. glabrata snails after infections 929 The survival rates of B. glabrata subjected to infection by different S. mansoni strains were 930 observed over 14 weeks. Kaplan Meier graphs were generated using xlstat, and the log-rank 931 test (p < 0.05) was used to test for significant between-group differences. Colored curves 932 indicate the mortality rates of naïve snails (yellow) (n=60), Supporting Information Legends 920 921 S1 Fig: Clustering of all differential represented transcripts 922 Clustering of differentially represented transcripts, p.934

/. Bgbre and . Smven, The difference in mortality between naïve and infected snails 935 was significant (p<0.05), whereas that between the two infected conditions was not (p = 0.243). 936 937 S3 Fig: Blast-like cells are non-proliferative cells

, In vitro EdU labeling of hemocytes collected for in vitro analysis, p.939

, EdU-labeled hemocytes from snails subjected to the allopatric interaction BgBRE/SmVEN at 940 24 h post-infection (BV24)

, Three individual snails were used for each condition. 944 Green label: EdU-positive cells; and blue label: DAPI. Magnification x63. 945 946 S1 Table: Biomphalaria and Schistosoma compatibility between African and South-American 947 strains 948 The prevalence (P %: percentage of snail infected) and intensity (I: number of parasites per 949 infected host) of infection are presented for each experimental infection. The indicated values 950 correspond to 10 miracidia, Blast-like cells were never labelled by EdU, indicating that these cells are not proliferative 943 when circulating in the hemolymphe, p.951

. Bgven, Biomphalaria pfeifferi from Senegal (BpSEN) and Schistosoma mansoni, p.952

. Smven, SmSEN) or Schistosoma rodhaini (Srod) were tested for compatibility. The 953 observation of cercariae shedding is also indicated. Cercariae shedding have been observed 954 between 35 and 38 days after miracidial infections NA: non-available data

R. Giardine, ) (defaults 961 settings) allows for quantifying the differential gene expression with comparing two biological 962 duplicates from uninfected snails sample (Bre1 and Bre2) against infected samples 963 (Pvalue<0.1). For each cluster transcript ID, Quality reads (Phred score >29) were aligned on the transcriptome assembly using the C++ 958 script Bowtie2, p.960, 2005.

, List of transcripts express by Schistosoma within Biomphalaria glabrata tissues 967 highlight in RNAseq clusters