Beyond plant defense: insights on the potential of salicylic and methylsalicylic acid to contain growth of the phytopathogen Botrytis cinerea

C. Dieryckx (1) , V. Gaudin (2) , J. W. Dupuy , M. Bonneu (3) , V. Girard (4, 1) , D. Job (1)

1 MAP - Microbiologie, adaptation et pathogénie
2 Laboratoire d'études et de recherches sur les médicaments vétérinaires et les désinfectants
3 PGFB - Plateforme génomique fonctionnelle
4 FungiPath - Génomique fonctionnelle des champignons pathogènes des plantes

Using Botrytis cinerea we confirmed in the present work several previous studies showing that salicylic acid, a main plant hormone, inhibits fungal growth in vitro. Such an inhibitory effect was also observed for the two salicylic acid derivatives, methylsalicylic and acetylsalicylic acid. In marked contrast, 5-sulfosalicylic acid was totally inactive. Comparative proteomics from treated vs. control mycelia showed that both the intracellular and extracellular proteomes were affected in the presence of salicylic acid or methylsalicylic acid. These data suggest several mechanisms that could potentially account for the observed fungal growth inhibition, notably pH regulation, metal homeostasis, mitochondria' respiration, ROS accumulation and cell wall remodeling. The present observations support a role played by the phytohormone SA and derivatives in directly containing the pathogen. Data are available via ProteomeXchange with identifier PXDO02873.

Domaines: Sciences du Vivant [q-bio]; Microbiologie et Parasitologie; Interactions entre organismes

C. Dieryckx, V. Gaudin, J. W. Dupuy, M. Bonneu, V. Girard, et al.. Beyond plant defense: insights on the potential of salicylic and methylsalicylic acid to contain growth of the phytopathogen Botrytis cinerea. Frontiers in Plant Science, 2015, 6, pp.859. ⟨10.3389/fpls.2015.00859⟩. ⟨hal-02001311⟩