Skip to Main content Skip to Navigation
Journal articles

Impact of human milk pasteurization on the kinetics of peptide release during in vitro dynamic digestion at the preterm newborn stage

Abstract : Holder pasteurization (62.5 °C, 30 min) of human milk denatures beneficial proteins. The present paper aimed to assess whether this can affect the kinetics of peptide release during digestion at the preterm stage. Raw (RHM) or pasteurized (PHM) human milk were digested in triplicates using an in vitro dynamic system. Mass spectrometry and multivariate statistics were conducted. Pre-proteolysis occurred mostly on β-casein, for which cumulative peptide abundance was significantly greater in PHM over 28% of the hydrolysed sequence. Eight clusters resumed the kinetics of peptide release during digestion, which differed on seven clusters (69% of the 1134 peptides). Clusters associated to the heat-denaturated proteins, lactoferrin and bile salt-stimulated lipase, presented different kinetics of release during digestion, unlike that for β-casein. Some bioactive peptides from β- casein presented significant different abundances between PHM and RHM before digestion (1–18, 185–211) or in during intestinal digestion (154–160, 161–166). Further physiological consequences should be investigated.
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-01984298
Contributor : Archive Ouverte Prodinra <>
Submitted on : Wednesday, January 16, 2019 - 8:08:52 PM
Last modification on : Wednesday, October 14, 2020 - 4:20:14 AM

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Citation

Amélie Deglaire, Samira Cassia de Oliveira, Julien Jardin, Valérie Briard-Bion, Florian Kroell, et al.. Impact of human milk pasteurization on the kinetics of peptide release during in vitro dynamic digestion at the preterm newborn stage. Food Chemistry, Elsevier, 2019, 281, pp.294-303. ⟨10.1016/j.foodchem.2018.12.086⟩. ⟨hal-01984298⟩

Share

Metrics

Record views

535