Discrimination of neutral oligosaccharides through a nanopore

Laurent Bacri (1) , Abdelghani Oukhaled (1) , Erven Hémon (1) , Fenseth Banzouzi Bassafoula (1) , Loïc Auvray (1) , Régis Daniel (1)

1 LAMBE - Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement

The detection of oligosaccharides at the single-molecule level was investigated using a protein nanopore device. Neutral oligosaccharides of various molecular weights were translocated through a single α-hemolysin nanopore and their nano-transit recorded at the single-molecule level. The translocation of maltose and dextran oligosaccharides featured by 1 → 4 and 1 → 6 glycosidic bonds respectively was studied in an attempt to discriminate oligosaccharides according to their polymerization degree and glycosidic linkages. Oligosaccharides were translocated through a free diffusion regime indicating that they adopted an extended conformation during their translocation in the nanopore. The dwell time increased with molecular mass, suggesting the usefulness of nanopore as a molecular sizing device.

Mots clés: Nanopore; α-Hemolysin; Oligosaccharides; Single molecule; Translocation

Domaines: Chimie analytique; Biophysique

Laurent Bacri, Abdelghani Oukhaled, Erven Hémon, Fenseth Banzouzi Bassafoula, Loïc Auvray, et al.. Discrimination of neutral oligosaccharides through a nanopore. Biochemical and Biophysical Research Communications, 2011, 412 (4), pp.561-564. ⟨10.1016/j.bbrc.2011.07.121⟩. ⟨hal-01967554⟩