Effect of titanium dioxide film thickness on photocatalytic and bactericidal activities against Listeria monocytogenes

Abstract : Structural, microstructural and bactericidal surface properties of TiO2‐coated glass substrates elaborated by reactive RF‐sputtering are investigated. As pathogenic bacteria in biofilms are a major concern in food industries due to their growing resistance to cleaning and sanitizing procedures, the development of photoactive surfaces exhibiting bactericidal properties is acknowledged as an effective approach to tackle bacterial contaminations. Our principal aim concerns the study of the photoactive top‐layer thickness impact (from 80 nm to ~500 nm) on Listeria monocytogenes. Structural characterization of the TiO2 layers demonstrates that anatase and rutile phases are both present, depending on the film thickness. Photocatalytic activity of the samples has been evaluated through the degradation of aqueous methylene blue (MB) solutions under UVA light illumination for various time periods. The results show an efficiency rating increase according to TiO2 film thickness up to a threshold value close to 400 nm. Moreover a significant decrease of the adherent bacteria number is observed after 20 min of UVA illumination. The quantitative study of the bactericidal activity associated with scanning electron microscopy observations of the post‐process bacteria damaged cells demonstrates the efficiency of the 240 nm‐thick TiO2 coating sample. The results are correlated with the production of hydroxyl radical during the process of photocatalysis.
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Contributor : Xavier Castel <>
Submitted on : Monday, January 14, 2019 - 11:33:28 AM
Last modification on : Tuesday, February 19, 2019 - 3:20:02 PM


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Marion Barthomeuf, Xavier Castel, Laurent Le Gendre, Justine Louis, Martine Denis, et al.. Effect of titanium dioxide film thickness on photocatalytic and bactericidal activities against Listeria monocytogenes. Photochemistry and Photobiology, Wiley, In press, ⟨10.1111/php.13078⟩. ⟨hal-01966652⟩



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