Live nanoscopic to mesoscopic topography reconstruction with an optical microscope for chemical and biological samples

Olivier Theodoly (1) , Nicolas Garcia-Seyda (1) , Frédéric Bedu (2) , Xuan Luo (1) , Sylvain Gabriele (3) , Tam Mignot (4) , Joanna Giermanska (5) , Jean-Paul Chapel (6) , Mélinda Métivier (7) , Marie-Pierre Valignat (1)

1 LAI - Adhésion et Inflammation
2 CINaM - Centre Interdisciplinaire de Nanoscience de Marseille
3 MNRS - MateriaNova Research Center
4 LCB - Laboratoire de chimie bactérienne
5 CRPP - Centre de recherches Paul Pascal
6 CRPP - Centre de Recherche Paul Pascal
7 Nanolane

Macroscopic properties of physical and biological processes like friction, wetting, and adhesion or cell migration are controlled by interfacial properties at the nanoscopic scale. In an attempt to bridge simultaneously investigations at different scales, we demonstrate here how optical microscopy in Wet-Surface Ellipsometric Enhanced Contrast (Wet-SEEC) mode offers imaging and measurement of thin films at solid/liquid interfaces in the range 1-500 nm with lateral optical resolution. A live, label-free and noninvasive methodology integrated with microfluidic devices allowed here characterization of polymers and proteins patterns together with corresponding phenotypes of living cells.

Domaines: Optique [physics.optics]; Science des matériaux [cond-mat.mtrl-sci]; Sciences du Vivant [q-bio]

Olivier Theodoly, Nicolas Garcia-Seyda, Frédéric Bedu, Xuan Luo, Sylvain Gabriele, et al.. Live nanoscopic to mesoscopic topography reconstruction with an optical microscope for chemical and biological samples. PLoS ONE, 2018, 13 (12), pp.e0207881. ⟨10.1371/journal.pone.0207881⟩. ⟨hal-01960422⟩