Specific labeling of mitochondria of Chlamydomonas with cationic helicene fluorophores

Christoph Bauer , Romain Duwald (1) , Geraldine Maria Labrador , Simon Pascal (2) , Pau Moneva Lorente , Johann Bosson (3) , Jérôme Lacour , Jean-David Rochaix (1)

1 UNIGE - Université de Genève = University of Geneva
2 CINaM - Centre Interdisciplinaire de Nanoscience de Marseille
3 Département de chimie physique [Genève]

Twelve cationic helicenes and one triangulene were tested for the specific labeling of mitochondria from algal cells. Octyl ester derivative 5 readily penetrates algal cells and gives rise to clear fluorescence patterns when it is used at concentrations in the μM range. Under these conditions, cell structures are well preserved and cell survival is not compromised. Cationic helicene compounds such as 5 provide new useful tools for examining the mitochondrial network and its dynamics including fission and fusion events.

Domaines: Chimie organique; Chimie thérapeutique; Sciences du Vivant [q-bio]

Christoph Bauer, Romain Duwald, Geraldine Maria Labrador, Simon Pascal, Pau Moneva Lorente, et al.. Specific labeling of mitochondria of Chlamydomonas with cationic helicene fluorophores. Organic & Biomolecular Chemistry, 2018, 16 (6), pp.919-923. ⟨10.1039/C7OB02906C⟩. ⟨hal-01957770⟩