Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana Using Chemical Genetics.

Clémence Bonnot (1, 2, 3) , Laurent Nussaume (2, 3) , Thierry Desnos (2, 3)

1 IAM - Interactions Arbres-Microorganismes
2 BIAM - Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB)
3 PEPSS - Plant Environmental Physiology and Stress Signaling

In spite of its importance for agriculture and 30 years of genetic studies, the phosphate-starvation signaling pathway, that allows plants to detect, respond, and adapt to changes in the phosphate concentration of the rhizosphere, remains poorly known. Chemical genetics has been increasingly and successfully used as a complementary approach to genetics for the dissection of signaling pathways in diverse organisms. Screens can be designed to identify chemicals interfering specifically with a pathway of interest. We designed a screen that led to the discovery of the first chemical capable to induce specifically the phosphate-starvation signaling pathway in Arabidopsis thaliana. This procedure, described here, can be adapted for the discovery of inducers or repressors of other pathways.

Mots clés: Phosphate-starvation signaling pathway; Reporter gene; Structure–activity relationship; Mass-spectrometry; Metabolization; Degradation; Chemical genetics screen

Mots clés: arabidopsis thaliana

Domaines: Autre [q-bio.OT]

Clémence Bonnot, Laurent Nussaume, Thierry Desnos. Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana Using Chemical Genetics.. Plant Chemical Genomics, 1795, Humana Press, 242 p., 2018, Methods in Molecular Biology, 978-1-4939-7874-8. ⟨10.1007/978-1-4939-7874-8_6⟩. ⟨hal-01954699⟩