Blocking Stemness and Metastatic Properties of Ovarian Cancer Cells by Targeting p70S6K with Dendrimer Nanovector-Based siRNA Delivery
Résumé
Metastasis is the cause of most (>90%) cancer deaths and currently lacks effective treatments. Approaches to understanding the biological process, unraveling the most effective molecular target(s), and implementing nanotechnology to increase the therapeutic index are expected to facilitate cancer therapy against metastasis. Here, we demonstrate the potential advantages of bringing these three approaches together through the rational design of a small interfering RNA (siRNA) that targets p70 S6K in cancer stem cells (CSCs) in combination with den-drimer nanotechnology-based siRNA delivery. Our results demonstrated that the generation 6 (G 6) poly(amidoamine) dendrimer can be used as a nanovector to effectively deliver p70 S6K siRNA by forming uniform dendriplex nanoparticles that protect the siRNA from degradation. These nanoparticles were able to significantly knock down p70 S6K in ovarian CSCs, leading to a marked reduction in CSC proliferation and expansion without obvious toxicity toward normal ovarian surface epithelial cells. Furthermore, treatment with the p70 S6K siRNA/G 6 dendriplexes substantially decreased mesothelial interaction, migration and invasion of CSCs in vitro, as well as tumor growth and metastasis in vivo. Collectively, these results suggest that p70 S6K constitutes a promising therapeutic target, and the use of siRNA in combination with nanotech-nology-based delivery may constitute a new approach for molecularly targeted cancer therapy to treat metastasis.
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