Oriented Immobilization of [NiFeSe] Hydrogenases on Covalently and Noncovalently Functionalized Carbon Nanotubes for H2/Air Enzymatic Fuel Cells

Solène Gentil (1, 2) , Syamim Muhamad Che Mansor (1) , Jamet Hélène (1) , Serge Cosnier (1) , Christine Cavazza (2) , Alan Le Goff (1)

1 DCM - Département de Chimie Moléculaire
2 LCBM - UMR 5249 - Laboratoire de Chimie et Biologie des Métaux

We report the oriented immobilization of [NiFeSe] hydrogenases on both covalently and noncovalently modified carbon nanotubes (CNTs) electrodes. A specific interaction of the [NiFeSe] hydrogenase from Desulfomicrobium baculatum with hydrophobic organic molecules was probed by electrochemistry, quartz crystal microbalance with dissipation monitoring (QCM-D), and theoretical calculations. Taking advantage of these hydrophobic interactions, the enzyme was efficiently wired on anthraquinone and adamantane-modified CNTs. Because of rational immobilization onto functionalized CNTs, the O-2-tolerant [NiFeSe]-hydrogenase is able to efficiently operate in a H-2/air gas-diffusion enzymatic fuel cell.

Mots clés: hydrogenases; carbon nanotubes; diazonium; biofuel cells; hydrogen oxidation

Domaines: Catalyse; Matériaux

Solène Gentil, Syamim Muhamad Che Mansor, Jamet Hélène, Serge Cosnier, Christine Cavazza, et al.. Oriented Immobilization of [NiFeSe] Hydrogenases on Covalently and Noncovalently Functionalized Carbon Nanotubes for H2/Air Enzymatic Fuel Cells. ACS Catalysis, 2018, 8 (5), pp.3957-3964. ⟨10.1021/acscatal.8b00708⟩. ⟨hal-01944392⟩