Cold Atmospheric Plasma Parameters Investigation for Drug Delivery Applications

Abstract : Cold atmospheric plasmas (CAPs) have demonstrated to be quite useful tools in biomedical applications and their relevance in medicine is getting more and more recognized. Drug delivery in cancer cells combining low cytotoxicity and high efficiency remains to be optimized with physical techniques. Several studies have shown that plasma could be used to deliver chemotherapy [1] or nucleic acids [2] via enhancement of cell permeabilization. We investigated the parameters needed for helium plasma to induce cell membrane permeabilization on cancer cells for drug delivery purposes. 10,000 plasma pulses were used at a frequency of 100 Hz for 100 s with an applied voltage of 14 kV. Both plasma electric field and endocytosis involvement in molecular uptake were also studied [3]. Kinetics of plasma-induced permeabilization were studied. Highest permeabilization efficiency was observed when propidium iodide was added after treatment (up to 40% of propidium iodide positive cells in both HeLa and 4T1). No significant toxicity was observed in these conditions as the viability in HeLa cells was measured to be of 80%. Doxorubicin was used as an anticancer drug. A treatment combining doxorubicin and plasma decreased the concentration of doxorubicin needed to achieve 50% of cytotoxicity by enhancing doxorubicin uptake in cancer cells. References [1] W. Zhu, S.-J. Lee, N. J. Castro, D. Yan, M. Keidar, and L. G. Zhang, Scientific Report, 6, (2016). [2] M. Jinno, Y. Ikeda, H. Motomura, Y. Kido and S. Satoh. Archives Biochemistry Biophysics, 605, 59-66 (2016) [3] V. Vijayarangan, A. Delalande, S. Dozias, J.-M. Pouvesle, C. Pichon, and E.
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Contributor : Jean-Michel Pouvesle <>
Submitted on : Monday, December 3, 2018 - 2:57:41 PM
Last modification on : Friday, May 24, 2019 - 11:36:03 AM


  • HAL Id : hal-01942869, version 1



Vinodini Vijayarangan, Anthony Delalande, Sébastien Dozias, Jean-Michel Pouvesle, Chantal Pichon, et al.. Cold Atmospheric Plasma Parameters Investigation for Drug Delivery Applications. ICPM7, ISPM, Jun 2018, Philadelphia, United States. ⟨hal-01942869⟩



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