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Article Dans Une Revue Methods in Molecular Biology Année : 2016

Lipid Homeostasis and Ligands for Liver X Receptors: Identification and Characterization.

Résumé

Screening of bona fide ligands for nuclear receptors is a real tour de force as the identified molecules are supposed to be able to activate the targeted proteins in cell culture as well as in vivo. Indeed orphan nuclear receptors are putative pharmacologically targets for various diseases. It is thus necessary to have quick and reproductive systems that help in identifying new ligands, agonist or antagonist, before using them in vivo in animal models to check for secondary effects. Here, we describe the transient transfections (homologous and heterologous) used for the screening of ligands for liver X receptor alpha (LXRalpha, NR1H3) in HeLa cells.
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Dates et versions

hal-01939754 , version 1 (29-11-2018)

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A. Lobaccaro, Claude Beaudoin, Bagora Bayala, Silvere Baron, Amalia Trousson. Lipid Homeostasis and Ligands for Liver X Receptors: Identification and Characterization.. Methods in Molecular Biology, 2016, 1443, pp.13-9. ⟨10.1007/978-1-4939-3724-0_2⟩. ⟨hal-01939754⟩
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